BIOL5393M Bioscience MSc Research Project Example Solution PDF | University of Leeds

Chapter 1: Introduction

1.1 Background of the Study

The advanced technique called mass spectrometry (MS) has proved to be an efficient method in the analysis of biomolecules, and more specifically, the tertiary and quaternary structures of proteins, DNA and RNA. The ability to present high-quality mass-tocharge ratios of biomolecules through the help of MS enables analysis of the molecular compositions as well as their interactions. In the context of structural biology, IM-MS and UVPD provide valuable structural information and complement existing methods of biomolecular analysis by characterizing the conformational states of biomolecules, patterns of protein folding, as well as interactions of proteins and ligands[1]. This contrasts with the X-ray crystallography and NMR, which are capable of analysing proteins in their native state, or the state that is the closest to the natural state possible, and this is definitely possible when studying proteins as these are required to function biologically............View More 

Table of Contents

Chapter 1: Introduction ..................................................................................................................................................................... 2
1.1 Background of the Study ......................................................................................................................................................... 2
1.2 Problem Statement .................................................................................................................................................................. 3
1.3 Research Aim and Objectives .................................................................................................................................................. 3
1.4 Significance of the Study......................................................................................................................................................... 3
1.5 Scope of the Study................................................................................................................................................................... 4
Chapter 2: Method ............................................................................................................................................................................ 4
2.1 Research Design...................................................................................................................................................................... 4
2.2 Instruments and Techniques..................................................................................................................................................... 4
2.2.1 Waters Synapt G1 for Ion Mobility Mass Spectrometry (IM-MS) ...................................................................................... 4
2.2.2 Thermos Orbitrap Eclipse for Ultraviolet Photodissociation (UVPD) ................................................................................. 5
2.3 Sample Preparation.................................................................................................................................................................. 6
2.4 Experimental Procedure........................................................................................................................................................... 6
2.4.1 Ion Mobility Mass Spectrometry (IM-MS) Procedure ........................................................................................................ 6
2.4.2 Ultraviolet Photodissociation (UVPD) Procedure .............................................................................................................. 7
2.5 Data Analysis.......................................................................................................................................................................... 7
2.6 Validity and Reliability............................................................................................................................................................ 7
Chapter 3: Results............................................................................................................................................................................. 8
Chapter 4: Discussion ..................................................................................................................................................................... 12
4.1 Analysis of Findings.............................................................................................................................................................. 12
4.2 Novel Observations............................................................................................................................................................... 12
4.3 Future Work.......................................................................................................................................................................... 12
Reference ....................................................................................................................................................................................... 13

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