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Talk to an Expert| Category | Assignment | Subject | Education |
|---|---|---|---|
| University | Kingstone University London | Module Title | CH7040 Specialised Analytical Techniques |
| Word Count | <800 Words |
|---|---|
| Assessment Title | M404 SDS-PAGE |
| Academic Year | 2026 |
Each practical report will have a 10% weighting and a maximum word count of <800 words. Each report will be marked with a total of 20 marks.
The Data, Results and Discussion sections should include: chemical reactions (if any), relevant equations (if any), calculations, the results obtained, schemes, data, observations, comments and discussions where scientific literature can be cited. Scanned experimental data, such as chromatograms or spectra, will need to be uploaded to Canvas.
(There may be some variation in marking if it is not possible to carry out certain parts of the experiment.)
1: For your report, you must include both images (annotated appropriately) detailing the 7.5% and 15% Coomassie-stained acrylamide gels. (8 marks)
Marks for correct annotations.
2: Discuss why cellular proteins in the cellular lysate can migrate in an electric field. (4 marks)
Marks for correct discussion of migration.
3: Discuss why the separation profile of stained cellular proteins in the 2 gels appears different. (4 marks)
Marks for explaining why they are different.
4: Discuss the variety of applications that SDS-PAGE may address in medical research laboratories. (4 marks)
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