Human Genome Project Laboratory Methods Tips For PCR Primers Design

Published: 28 Jul, 2025
Category Assignment Subject Education
University Module Title Human Genome Project Laboratory Methods

HGP Tips For PCR Primers Design

  1. Aim for the GC content to be between 40 and 60% with the 3’ of a primer ending in G or C to promote binding. This is known as a GC Clamp. The G and C bases have stronger hydrogen bonding and help with the stability of the primer. Be mindful not to have too many repeating G or C bases, as this can cause primer-dimer formation.
  2. A good length for PCR primers is generally around 18-30 bases. Specificity usually is dependent on length and annealing temperature. The shorter the primers are, the more efficiently they will bind or anneal to the target.
  3. Try to make the melting temperature (Tm) of the primers between 65°C and 75°C, and within 5°C of each other. Because the Tm is dependent on the length, it’s important to keep primers on the shorter end. The bases also impact the Tm, G and C result in higher melting temperatures than A and T. If the Tm of your primer is very low, try to find a sequence with more GC content, or extend the length of the primer a little.
  4. Typically, 3 to 4 nucleotides are added 5 ’of the restriction enzyme site in the primer to allow for efficient cutting.
  5. Try to avoid regions of secondary structure and have a balanced distribution of GC-rich and AT-rich domains.
  6. Try to avoid runs of 4 or more of one base, or dinucleotide repeats (for example, ACCCC or ATATATAT).
  7. Avoid intra-primer homology (more than 3 bases that complement within the primer) or inter-primer homology (forward and reverse primers having complementary sequences). These circumstances can lead to self-dimers or primer-dimers instead of annealing to the desired DNA sequences.

Hire Experts to solve this Assignment before your Deadline

Pay & Buy Non Plagiarized Assignment

 

If you are falling short of time left and searching for expert help for your Human Genome Project Laboratory Methods Assignment? Our team of professional diploma writers provides top-quality Best Assignment Help. Just write my assignment, and your assignment will be completed on time. We are ensuring you get the best results on our affordable Management Assignment Help. Contact us today for a plagiarism-free assignment solutions. This will help you stand out from the rest with better grades.

Workingment Unique Features

Hire Assignment Helper Today!


Latest Free Samples for University Students

RBP020L063H Leadership and Change Management Assignment Sample

Category: Assignment

Subject: Management

University: University of Roehampton

Module Title: RBP020L063H Leadership and Change Management

View Free Samples

HRMM080 Ethical and Responsible Leadership AS2 Reflective Portfolio Sample

Category: Assignment

Subject: Management

University: University of Northampton

Module Title: HRMM080 Ethical and Responsible Leadership

View Free Samples

ACAD1346 The child’s live Experience Developing Confidence Learners Assignment Sample

Category: Assignment

Subject: Education

University: University of Greenwich (UOG)

Module Title: ACAD1346 The child’s live Experience Developing Confidence Learners

View Free Samples

NUR7011 Developing Healthcare Leaders Assignment Sample | BPP

Category: Assignment

Subject: Nursing

University: BPP University

Module Title: NUR7011 Developing Healthcare Leaders

View Free Samples

Project Management, Leadership and Skills: Planning & Control Portfolio Example

Category: Assignment

Subject: Management

University: University of Salford Manchester

Module Title: Project Management, Leadership and Skills: Planning & Control

View Free Samples
Online Assignment Help in UK